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mruby2 559 600 113  (Addgene inc)


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    Addgene inc mruby2 559 600 113
    Mruby2 559 600 113, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mruby2 559 600 113/product/Addgene inc
    Average 93 stars, based on 86 article reviews
    mruby2 559 600 113 - by Bioz Stars, 2026-03
    93/100 stars

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    A Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with <t>mAID/mRuby2.</t> B Order of events for the generation of the different cell lines. C Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. Experiments in each panel were performed at least three times, and the representative results are shown. D Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. E Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (* p < 0.05). Data are presented as mean values +/− SEM from biological triplicates. Source data are provided as a Source Data file.
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    A Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. B Order of events for the generation of the different cell lines. C Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. Experiments in each panel were performed at least three times, and the representative results are shown. D Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. E Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (* p < 0.05). Data are presented as mean values +/− SEM from biological triplicates. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells

    doi: 10.1038/s41467-024-47314-4

    Figure Lengend Snippet: A Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. B Order of events for the generation of the different cell lines. C Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. Experiments in each panel were performed at least three times, and the representative results are shown. D Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. E Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (* p < 0.05). Data are presented as mean values +/− SEM from biological triplicates. Source data are provided as a Source Data file.

    Article Snippet: In order to incorporate mRuby2, we replaced mCherry2 in the donor plasmid (Addgene #121180).

    Techniques: CRISPR, Western Blot, Biomarker Discovery, Fluorescence, DNA Methylation Assay, Liquid Chromatography with Mass Spectroscopy